Cell Isolation and Tissue Culture Sub Core

Function/ Services of the CORE:

The function of the Cell Isolation and Tissue Culture Core is to provide efficient, economical, and reproducible isolation of pulmonary and cardiac endothelial cells, fibroblasts, vascular smooth muscle cells, epithelial cells, macrophages; propagation, and phenotypic characterization and function.

One of the greatest difficulties for investigators working with various primary lung and heart cell populations is obtaining consistently viable, highly purified, cell sub-populations for ex vivo analyses from cardiopulmonary sources in an efficient and reproducible manner. And while each investigator in this COBRE has a varying degree of expertise in such cell isolations, a centralized resource for the isolation, propagation, and characterization of cells from the heart and lung are fundamentally important services for all Investigators, and foundational to the success of the CPVB COBRE.

Organ Function Sub Core

Function/ Services of the CORE:

To provide measurement of heart, lung, and vessel function, in vivo angiogenesis, and transient gene manipulation in vivo. The COBRE Core will provide expertise and state of the art equipment for assessing heart, lung, and vessel function, in vivo angiogenesis, and transient gene manipulation in vivo. The Cell/Organ Core has been measuring heart and lung function using echocardiogram and Flexivent™ methodological approach. The Core has developed the liposome-mediated introduction of cDNA into mouse lungs and assesses in vivo angiogenesis. Both of these techniques are available to users upon request. Further, since purchasing the laser Doppler imaging equipment, the Core has been performing vessel flow.

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