Cell Isolation Services

Primary cell isolation (mouse, rat, pig, human), characterization, propagation, phenotyping from endothelial cells (heart, lung, vessel); fibroblasts (heart); epithelial cells (lung)
Electrical cell impedance system (ECIS)
Enzyme-linked immunosorbent assay (ELISA)
Flow cytometry
Mitochondrial respiration/glycolysis measurements (Seahorse)
Brightfield and fluorescent imaging using BioTek Cytation 5
Endothelial cell function
- Proliferation, migration, adhesion, tube formation, permeability, apoptosis-susceptibility assays
Frozen tissue immunohistochemistry
- Cryosectioning, tissue staining (IHC/IF)
Aperio brightfield and fluorescent scanning and image analysis

Organ Function Services

Invasive in vivo lung hemodynamics
- Flexivent
- Capillary filtration coefficient
Liposome injection
- cDNA, siRNA
Rodent imaging services
- Echocardiogram
- Laser Doppler System
In vivo angiogenesis (Matrigel pouch)

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Testimonials

The Cell Isolation & Organ Function core is an incredible group of professionals who ensure that your hypothesis is tested with appropriate experiments, done with pristine technique, and the results are ready for analysis and publication.

Sean Monaghan, MD

The Cell Isolation and Organ Function Core has been a tremendous asset to my research program. The Core uses highly rigorous approaches, delivers timely results, and core staff are collaborative and easy to work with. I would highly recommend this resource to investigators!

Corey E. Ventetuolo, MD, MS

The Cell Isolation & Organ Function Core has made it possible for us to perform echocardiograms in adult zebrafish. The ability to perform echos in adult fish has opened up a whole new realm of research for my lab and is facilitating our ability to ask important research questions about the cellular and molecular mechanisms mediating cardiac development and adult heart health.

Jessica Plavicki, PhD

The Cell Isolation & Organ Function core helps me isolate and characterize lung microvascular endothelial cells from neonatal and adult mice. The turnaround time is about a week. This is a reliable service which moves my research forward smoothly.

Hongwei Yao, PhD

I am a junior investigator and a COBRE alumnus. I have been working with the CPVB Cell Isolation & Organ Function Core since 2015. Everyone in the Core recognizes the importance of offering high quality and timely services that would greatly facilitate collaborative research within our community. I appreciate the great diligence and efficiency that the Core has provided over the years.

Yang Zhou, PhD

The Cell Isolation & Organ Function core has provided wonderful support for my research and it has been instrumental for getting preliminary data for launching my independent research program. I have used the brightfield and fluorescent imaging and scanning services (Biotek Cytation 5 and Aperio Scanscope) for assessing angiogenesis and immune responses in vitro and in vivo. The staff members are always professional and courteous, and their work is always timely and accurate. Thank you for the great service you provide!

Fabiola Munarin, PhD

Cell Isolation Services

Organ Function Services

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Ocean State Research Institute

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