Cell Isolation Services

  • Primary cell isolation (mouse, rat, pig, human), characterization, propagation, phenotyping from endothelial cells (heart, lung, vessel); fibroblasts (heart)
  • Electrical cell impedance system (ECIS)
  • Enzyme-linked immunosorbent assay (ELISA)
  • Flow cytometry
  • Mitochondrial respiration/glycolysis measurements (Seahorse)
  • Brightfield and fluorescent imaging using BioTek Cytation 5
  • Endothelial cell function
    • Proliferation, migration, adhesion, tube formation, permeability, apoptosis-susceptibility assays
  • Frozen tissue immunohistochemistry
    • Cryosectioning, tissue staining (IHC/IF)
  • Aperio brightfield and fluorescent scanning and image analysis

Organ Function Services

  • Invasive in vivo lung hemodynamics
    • Flexivent
    • Capillary filtration coefficient
  • Liposome injection
    • cDNA, siRNA
  • Rodent imaging services
    • Echocardiogram
    • Laser Doppler System
  • In vivo angiogenesis (Matrigel pouch)

Respiratory Services

  • Cigarette smoke model and chronic hypoxia model
  • Lung and heart structural and vascular remodeling assessments
  • Mitochondria isolation and physiological/biochemical assays
  • Mitochondrial respiration and function
    • Seahorse XFe96 Extracellular Flux Analyzer
    • High-resolution O2k-FluoRespirometers (Oroboros)