Cell Isolation and Organ Function Services

CIOF- Core Director: Hongwei Yao, PhD (Hongwei_Yao@brown.edu)
CIOF- Core Manager: Valeria Zarate (valeria_zarate@brown.edu)

  • CIOF Personnel
  • Aperio brightfield and fluorescent scanning and image analysis
  • Brightfield and fluorescent imaging using BioTek Cytation 5
  • Primary cell isolation (mouse, rat, pig, human), characterization, propagation, phenotyping from endothelial cells (heart, lung, vessel); fibroblasts (heart)
  • Electrical cell impedance system (ECIS)
  • Endothelial cell function
    • Proliferation, migration, adhesion, tube formation, permeability, apoptosis-susceptibility assays
  • Cell Transfection/Transduction/Infection
  • Frozen tissue immunohistochemistry
    • Cryosectioning, tissue staining (IHC/IF)
  • Tissue Morphometry
  • Lung or Heart ex vivo Measurements
  • Data Analysis

Mitochondrial Function and In Vivo Imaging (MF-II) Services

MF-II Core Director: Peng Zhang, MD, MS (peng_zhang@brown.edu)
MF-II Core Manager: Valeria Zarate (valeria_zarate@brown.edu)

  • MF-Il Personnel
  • Echocardiography
  • Seahorse XFe96 Extracellular Flux Analyzer
  • High-resolution O2k-FluoRespirometers (Oroboros)
  • Laser Doppler System
  • Hemodynamics
  • Micro-CT
  • PET
  • Cigarette smoke model and chronic hypoxia model
  • Treadmill