Cell Isolation and Organ Function Services
CIOF- Core Director: Hongwei Yao, PhD (Hongwei_Yao@brown.edu)
CIOF- Core Manager: Valeria Zarate (valeria_zarate@brown.edu)
- CIOF Personnel
- Aperio brightfield and fluorescent scanning and image analysis
- Brightfield and fluorescent imaging using BioTek Cytation 5
- Primary cell isolation (mouse, rat, pig, human), characterization, propagation, phenotyping from endothelial cells (heart, lung, vessel); fibroblasts (heart)
- Electrical cell impedance system (ECIS)
- Endothelial cell function
- Proliferation, migration, adhesion, tube formation, permeability, apoptosis-susceptibility assays
- Cell Transfection/Transduction/Infection
- Frozen tissue immunohistochemistry
- Cryosectioning, tissue staining (IHC/IF)
- Tissue Morphometry
- Lung or Heart ex vivo Measurements
- Data Analysis
Mitochondrial Function and In Vivo Imaging (MF-II) Services
MF-II Core Director: Peng Zhang, MD, MS (peng_zhang@brown.edu)
MF-II Core Manager: Valeria Zarate (valeria_zarate@brown.edu)
- MF-Il Personnel
- Echocardiography
- Seahorse XFe96 Extracellular Flux Analyzer
- High-resolution O2k-FluoRespirometers (Oroboros)
- Laser Doppler System
- Hemodynamics
- Micro-CT
- PET
- Cigarette smoke model and chronic hypoxia model
- Treadmill